WebInfected cells were screened with 5 µg/mL puromycin (Solarbio life Science, Beijing, China) for more than 1 week, and transfection efficiency was detected by Western blots. Western Blot Assays. Whole-cell lysates were prepared to extract the total protein, which was separated by 10% PAGE and transferred to a polyvinylidene fluoride membrane. WebApr 11, 2024 · The concentration of Ara-C was analyzed with reversed-phase high performance liquid chromatography (RP-HPLC, Shimadzu SIL-20A, Kyoto, Japan) using a C18 column (4.6 mm × 250 mm, 5 μm particle size, DIKMA, Beijing, China) with a linear gradient of acetonitrile containing 0.1% TFA from 0 to 100% in 10 min at a flow rate of 1.0 …
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http://english.ivpp.cas.cn/ WebJan 16, 2024 · The mitochondrial membrane potential was evaluated using a JC-1 Kit (Solarbio Life Science, Beijing, China), according to the manufacturer’s instructions. … curly gurl luv beauty supply
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WebApr 12, 2024 · For immunoblotting, the brain part of the zebrafish larvae was cut and lysed on ice with RIPA lysis buffer (R0010, Solarbio, Beijing, China) containing 1% phenylmethylsulfonyl fluoride (PMSF) (P0100, Solarbio), followed by tissue homogenate at 70 Hz for 90 s twice before being centrifuged at 12 000g for 20 min at 4°C to remove … WebJul 31, 2024 · School of Life Sciences, Beijing University of Chinese Medicine, Beijing, 102488, ... Journal of Traditional Chinese Medical Sciences, 2024, 9(4): ... We thank Prof. Feng Shao at the National Institute of Biological Sciences (Beijing, China) for the GSDMB, GSDMD plasmids, ... WebAnnual Data. Statistical Database. Monthly Data; Quarterly Data; Annual Data; Census Data curly green eyes