Psin-ef2 vector

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August undefined, 2024

WebMay 11, 2024 · abstract type AbstractPolynomial end """ Polynomial <: AbstractPolynomial Polynomials written in the canonical form --- Polynomial(v::T) where T<:Union{Vector{<:Real}, NTuple{<:Any, <:Real}}) Construct a `Polynomial` from the list of the coefficients. The coefficients are assumed to go from power 0 in the ascending order. WebJan 7, 2024 · Product Name : pSin-EF2-Sox2-Pur article : bacterial resistance : Ampicillin cloning : growth notes : The psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector. origin : 37 pi : plasmid copy : SINF-EF-G (from Dr. Robert Hawley, George Washington University) was modified to make the lentiviral backbone.

Addgene: pSin-EF2-Nanog-Pur

WebJan 22, 2015 · The pri-miR-34c sequence was cloned into the lentiviral plasmid pSin-EF2-puromycin (Addgene, Cambridge, MA, USA); pSin-EF2-miR-34c or negative control empty … WebConstructs expressing eGFP from the pSin- EF2-GFP-Puro based vector were used in subsequent studies. A key bottleneck in many stem cell applications is the ability to identify, select or ... in loving memory planters

Addgene: pSin Helper

WebThe empty pSin-EF2 vector (lenti-vector) was used as a control. Observation of morphologic changes. The cells were seeded into six-well culture (Corning Incorporated, Corning, NY, USA) plates and transferred with miR-16 or miR-Ctrl. After incubation for 24 hours, the cellular morphology was observed by phase contrast microscope (Olympus America ... WebOct 5, 2024 · To determine that whether LASP1 is a direct target of miR-203a-3p, CNE-2 and SUNE-1 cells were co-transfected with miR-203a-3p mimic or miR-Ctrl (50 nM, RiboBio) and the pSin-EF2-puro-LASP1 (LASP1) or empty pSin-EF2-puro-Vector (Vector) (2 μg; Addgene, Cambridge, MA, USA). The cells were harvested for assays 48hs after transfection. WebAug 14, 2015 · zt085 腺病毒载体系统 pAd/BLOCK-iT-DEST RNAi Gateway Vector Invitrogen ... zt1273 pSin-EF2-puro-OCT4 . zt1274 pSIREN-retroQ . zt1275 psiSTRIKE . zt1276 pST1347-cas9-D10A . zt1277 PT/neo(2200) zt1278 PT2/GFP(2000) zt1279 PT2/mCherry(321) zt1280 PT2/RMCE-puDTK(1500) ... in loving memory poster templates

Fusarium wilt of cyclamen : early detection and biocontrol

MiR-34c suppresses tumor growth and metastasis in ... - Nature

WebNov 17, 2024 · GFP-human Med15 and TagRFP-human DYRK3 fusion protein were subcloned into the lentiviral expression vector pSin-EF2 by ClonExpress II One Step Cloning Kit (Vazyme Biotech, C112). Primers used to clone lentiviral vectors are provided in Additional file 6: Table S3. The human embryonic kidney 293T cell line was used as a host … WebApr 27, 2024 · The DAB2IP expression construct was generated by subcloning PCR-amplified full-length human DAB2IP complementary DNA into the pSin-EF2-puro lentiviral vector (Addgene, beijing, China) by EcoRI and NheI. pSin-EF2-puro-DAB2IP or the empty lentiviral vector and 2 packaging plasmids pM2.G and psPAX2 (Addgene, beijing, China) … in loving memory plate framesWebApr 6, 2024 · Briefly, the pSin-EF2-TIPE3-HA or empty vector, as well as the lentivirus packaging plasmids psPAX2 and pMD2.G, were co-transfected into 293FT cells using the calcium phosphate method. After... in loving memory picture maker

"WebJun 1, 2024 · The lentiviral expressing vector encoding human Oct4 (pSin-EF2-Oct4-Pur, Addgene plasmid 16579) was obtained from Addgene (Cambridge, MA) . The lentiviral control vector pSin-null was derived from pSin-EF2-Oct4-Pur as previously described . For knockdown experiments, pLKO.1-puro-based lentiviral vectors, including stem-loop … " - Psin-ef2 vector

Psin-ef2 vector

Figure 6. Systemic delivery of pSin-EF2-ELMO1-Pur …

WebThe pSin-EF2- GFP-Puro (EF1 a promoter) provided maximal GFP expression in both the undifferentiated cells as well as the day 10 differentiated cells with greater than 95% of … http://www.labbase.net/Supply/SupplyItems-4229347.html

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WebOct 19, 2024 · The pSin-EF2-puro-ZNF671-HA or pSin-EF2-puro-vector plasmids were obtained from Land. Hua Gene Biosciences (Guangzhou, China); pSin-EF2-puro-Vector … Web伟通生物现成基因表达载体库开放供应伟通生物根据基因功能研究需要,制备和积累近千个基因表达载体。其中大基因的序列都经过测序验证,与ncbi上的注册序列保持一致。这些载体既可以直接作为基因功能研究使用，也可以作为新构建载体的模板。伟通将部分常用的，不涉及第三方知识产权的表达 ...

WebFull Sequences from Depositor (1) Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between …

WebThe psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector. These plasmids were created by your colleagues. Please acknowledge the Principal … WebFeb 3, 2024 · The AKR1B10 CDS (coding DNA sequence) was cloned into the lentiviral expression vector pSIN-EF2-Puro (hereinafter referred to as pSIN-EF2) to construct the lentiviral vector pSIN-EF2/AKR1B10. The method to establish stable AKR1B10-expression cell line has been described previously 23. AKR1B10-shRNA stable transfection using …

WebpSin4-EF2-IRES-Pur (Search Vector Database) Backbone size w/o insert (bp) 7500 Vector type Mammalian Expression, Lentiviral Selectable markers Puromycin Growth in Bacteria … Comprehensive molecular biology blog covering topics such as plasmids, viral ve…

WebApr 27, 2024 · The PCR and sequencing analyses confirmed that the DAB2IP gene sequence was consistent with the sequence in GenBank (NCBI, NM_138709). Stably transfected … in loving memory potsWebEF2.-Se define el espacio v h y los elementos que serán base para tal espacio. Con la discretización anterior de 51 la cual se denota por 7 h se asocian la siguiente definición y los siguientes espacios de dimensi6n finita: Dejinici6n.-Si K E 7 h entonces sedefine a hK como el diámetro del triángulo K , esto es, la longitud del lado m ... in loving memory quoteWebApr 20, 2012 · Fig. 7 outlines the vector (pSin-EF2-TK-Puro), with TK driven by the EF1α promoter. hES H9 cells were treated with BMP4 , allowing for trophoblast differentiation. Cells were infected with either the SSEA4 or CD24 antibody conjugated m 168 pseudotyped virus bearing the pSin-EF2-TK-Puro lentiviral vector, five days post BMP4 addition and ... in loving memory praying hands svgWebJan 22, 2015 · The pri-miR-34c sequence was cloned into the lentiviral plasmid pSin-EF2-puromycin (Addgene, Cambridge, MA, USA); pSin-EF2-miR-34c or negative control empty pSin-EF2 vector was then co-transfected ... in loving memory poems for motherWebB: Schematic construction of vectors encoding soluble scFv C1 and scFv C1-N1N2. The amber stop codon between the scFv and gene III in pHEN 2 was removed by mutagenesis … in loving memory photo ornamentWebMay 15, 2024 · 42EF-P2MPB-F4 Sensor Block 3D CAD Model Library GrabCAD. Join 9,340,000 engineers with over 4,840,000 free CAD files Join the Community. The CAD files … in loving memory prayerWebNov 1, 2024 · The pSin-EF2-empty vector or pSin-EF2-CES2 plasmids and the lentivirus packaging plasmids (pMD2.G and psPAX2) were co-transfected into 293FT cells using the calcium phosphate method, as previously described [14]. Lentivirus particles were harvested and infected into the CAL33 and UM1 cells. The vector or CES2 stably transfected cells … in loving memory quotes for father