Glycoworks hilic μelution提取板
WebMar 27, 2024 · The sample was then cooled to room temperature and mixed with 0.8 mg RapiFluor-MS reagent. The labeling reaction time was 5 min at RT. Acetonitrile (ACN) was then added to a final concentration of 89.5% and the reaction was cleaned up using Glycoworks® HILIC μElution plate. http://www.bioon.com.cn/doc/showarticle.asp?newsid=51258
Glycoworks hilic μelution提取板
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WebWaters Corp GlycoWorks HILIC µElution Plate. Supplier: Waters Corp 186002780. This plate is ideal for removing contaminants like salts and detergents from hydrophilic … WebThis GlycoWorks HILIC µElution Plate is well suited for removing contaminants like salts and detergents from hydrophilic analytes, such as carbohydrates, prior to mass …
WebWorks HILIC μElution Plate. G1 and G1F isomers were summed for simplicity and the data is shown in Table 1. Conclusion The bioZen N-Glycan Clean-Up microelution plate is an appro-priate replacement for the Waters GlycoWorks µElution plates in the Waters GlycoWorks RapiFlour-MS glycan workflow solution. Labeled Glycan Waters GlycoWorks WebDec 18, 2013 · GlycoWorks工作流程适用于两种不同的SPE型式：一种适合于高通量需求(96孔μElution提取板)，另一种适用于低通量应用(1 cc小柱)。 最重要的是，结果显示：无论选择何种GlycoWorks SPE型式，都可获得极为相似的多聚糖图谱。
WebFeb 20, 2024 · 此外，GlycoWorks μElution提取板为96孔样式，这意味着它可用于高通量实验，或者在低通量实验中连续使用。 HILIC SPE去除色谱干扰 和 Rapi Fluor-MS N-糖试剂盒的其它方面一样，我们对该 GlycoWorks SPE步骤进行了深入评估。 WebThis plate is ideal for removing contaminants like salts and detergents from hydrophilic analytes, such as carbohydrates, prior to mass spectrometry analysis. It enables elution …
WebApr 29, 2024 · Here we provide a protocol for labeling using Waters GlycoWorks RapiFluor-MS N-Glycan Kit and subsequent exoglycosidase digestion. 3.2.1 Rapid Deglycosylation. …
WebFigure 5. Extraction of RapiFluor-MS labelled N-glycans by SPE with a GlycoWorks HILIC μElution plate. (A) A test mixture comprised of RapiFluor-MS labelled glycans from pooled human IgG and bovine fetuin (Labelled N-glycans from 0.4 μg glycoprotein, 10 μL injection of ACN/DMF diluted sample). (B) The test mixture after extraction by HILIC SPE. phys immediate careWebOct 2, 2024 · GlycoWorks HILIC μElution Plate by Waters p/n 186002780; GlycoWorks SPE Reagents – Automation by Waters p/n 186008747; Kit Components for the … physio 1110WebBelow are general guidelines for utilizing GlycoWorks HILIC μElution Plate for extracting, purifying and concentrating carbohydrates. A 96-well collection plate and a waste tray … physimed walk inWebpreviously, HILIC is an ideal mode for retaining glycans due to their highly polar nature. Therefore, a procedure has been optimised using a HILIC SPE process, involving a silica … physio 1WebGlycoWorks HILIC uElution Plate. Skip to Content. 01357 522 961. Quick Order. My Account. Register. physio 100 meerbuschWebFigure 3. HILIC-FLR Analysis of 2-AB Labeled Glycans released from the GlycoWorks Control Standard using an ACQUITY UPLC GST Amide (BEH Glycan), 1.7 μm, 2.1 x 150 mm Column. The left panel displays chromatograms obtained for samples processed using (A) GlycoWorks HILIC 96-well μElution Plate, Peaks detected by UNIFI processing are tool temporary disabled by cod3rWebGlycoWorks HILIC μElution Plate (Waters Corp.). Glycans were dried by vacuum centrifugation and stored at -20 °C. Prior to injection into the LC-MS, each sample was reconstituted in 9 μL of LCMS-grade water, 8 μL of DMF, and 21 μL of ACN sequentially. Eight microliters (8 μL) (20 %) was injected onto tool temperaturen auslesen