Boston university mouse proximal tubule
WebOct 1, 2012 · Boston University mouse proximal tubule cells (BUMPTs; clone 306; obtained from Drs. Wilfred Lieberthal and John Schwartz, Boston University School of Medicine, Boston, MA) and human proximal convoluted tubule cells (HPCTs; obtained from Dr. Ulrich Hopfer, Case Western Reserve University, Cleveland, OH) were grown … WebSep 12, 2024 · We first evaluated the expression of BNIP3 in Boston University mouse proximal tubule (BUMPT) cells that were subjected to OGD-R to mimic in vivo IR. Immunoblotting analysis showed a dramatic...
Boston university mouse proximal tubule
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WebFeb 11, 2016 · We describe the development and characterisation of two genetically engineered mouse lines that allow for spatial and temporal targeting of conditional mutations in either all tubular epithelial compartments ( Pax8 -CreER T2 transgenic mice) or specifically in proximal tubular epithelial cells ( Slc22a6 -CreER T2 knock-in mice). WebDec 21, 2024 · BUMPT (Boston University mouse proximal tubular) cells, the mouse proximal tubular cell line, were purchased from ATCC. Cells were maintained in a culture medium containing DMEM, 10% FBS, and antibiotics in a humidified chamber with 5% CO 2. For cell treatment, cells were seeded into 6-well plates or 96-well plates containing cell …
WebSep 12, 2024 · We first evaluated the expression of BNIP3 in Boston University mouse proximal tubule (BUMPT) cells that were subjected … WebNov 21, 2024 · However, GPR37L1 only tended to reduce 5-cytosine methylation, which could indicate that that the GPR37L1 effect on DNA methylation is gene-specific, e.g., Nhr3 (vide infra). NHE3 is expressed on the apical membrane of the renal proximal tubule, which is responsible for about 60% of the sodium reabsorption by the proximal tubule [57,58,59].
WebIn kidney proximal tubules of male mice the mitochondria are larger and more electron-lucent, ... Testosterone-Mediated Sexual Dimorphism of Mitochondria and Lysosomes in Mouse Kidney Proximal Tubules. Harold Koenig, Alfred Goldstone, ... and Departments of Neurology and Biochemistry, Northwestern University Medical School, Chicago, … WebApr 15, 2024 · Based on the accessibility of the known marker genes, we identified clusters representing nephron progenitors, endothelial cells, podocytes, proximal tubule segment 1 and segment 3 cells, loop...
WebApr 16, 2024 · In cultured mouse proximal tubule cells, NAM blocked TGF–β‐induced expression of fibrotic proteins, while it marginally suppressed the morphological changes induced by TGF‐β. NAM also suppressed the expression of pro‐inflammatory cytokines (eg MCP‐1 and IL‐1β) during TGF‐β treatment of these cells. the loki finaleWebMar 1, 2024 · RLDC Treatment in Boston University mouse proximal tubular cell line Cells. Boston University mouse proximal tubular cell line (BUMPT) cells were seeded in 35-mm dishes at a density of 0.2 × 10 6 cells/dish. After overnight growth, the cells were subjected to 4 days of RLDC treatment, which consisted of 7 hours of 2-μM cisplatin … ticketswest yakima stateWebFeb 21, 2024 · In Boston University mouse proximal tubule (BUMPT) cells, high-glucose (HG) treatment led to a significant decrease in exosome secretion, which was associated with specific downregulation of RAB27B, a key guanosine-5'-triphosphatase in exosome … ticketswest wickedWebJul 8, 2024 · 3 RESULTS. Nephrons at different developing stages were located in inverse sequence to nephrogenesis throughout the cortex of E17.5 kidneys (Figure 1).Newly formed nephrons, RVs and SSBs were located around the transition of the nephrogenic zone and superficial cortex, together with the terminal tips of UB (Figures 1a,b).According to the … ticketswest waWebJan 5, 2024 · The goal of the present study was to test the hypothesis that renal proximal tubule epithelial IR activation contributes to obesity-induced kidney injury. We used … the lokha ubud villas \u0026 spa baliWebJan 5, 2024 · We used kidney proximal tubule IR–knockout (KPTIRKO) mice with targeted deletion of the IR in the proximal tubule epithelial cell. Our findings reveal that activation of proximal tubular IR is required for obesity-induced kidney injury in mice. Results Characterization of KPTIRKO mice. KPTIRKO mouse progeny grew normally. tickets wfc 2022WebThe proximal tubule efficiently regulates the pH of the filtrate by secreting hydrogen ions (acid) into the tubule and reabsorbing approximately 80% of the filtered bicarbonate. Fluid in the filtrate entering the proximal … the loki streaming