Binary vector pcambia-1300

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http://www.biovector.net/product/1319.html WebpCAMBIA1300 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available …

pCAMBIA2301 Sequence and Map - SnapGene

WebThe vector pCAMBIA- 1300: SniOLP was digested with Eco RI enzyme and made blunt ended by end filling. This blunt-ended vector was used for cloning the blunt-ended Rs-AFP2 expression cassette. WebPcambia 1300 (Search Vector Database) Vector type Plant Expression Selectable markers Hygromycin Growth in Bacteria Bacterial Resistance (s) Kanamycin, 50 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert Gene/Insert name FLAG-Pup (E) Promoter 35S Tag / Fusion Protein Triple Flag Tag … it is to walk at times in central park

Agrobacterium tumefaciens- - ScienceDirect

WebSDS for the pCambia vectors are available by request from Marker Gene Technologies, Inc. Shipping and Storage The plant expression vectors are shipped at room temperature. Store all samples of the vector at –20 C or below, once resuspended. Products are guaranteed for six months from date of shipment when stored properly. Contents WebNov 25, 2024 · In addition, we tested the pCAMBIA 1300-PtHCA 2-1 promoter-GUS binary expression vector, which contains the GUS gene ORF under the control of Populus … WebpCambia1300 Plant Expression Vector General notes Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and no reporter gene to allow construction of user's own system. The pCambia1300 vector offers: High copy number in E.coli for high DNA yields neighbor\\u0027s legs

Marker-Free Transgenic Chrysanthemum Obtained by

WebA twin T-DNA system is a convenient strategy for creating selectable marker-free transgenic plants. The standard transformation plasmid, pCAMBIA 1300, was modified into a binary vector consisting of two separate T-DNAs, one of which contained the hygromycin phosphotransferase (hpt) marker gene. WebA binary vector was invented soon after it had been elucidated that crown gall tumorigenesis was caused by genetic transformation of plant cells with a piece of DNA, T-DNA for transferred DNA,... neighbor\u0027s kitchen 宇部WebProduct name pCambia1300 Plant Expression Vector General notes Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for … neighbor\u0027s landing sunrise beach mo

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Binary vector pcambia-1300

A novel binary T-vector with the GFP reporter gene for …

WebA. tumefaciens strains EHA 101 and EHA 105 contained the disarmed plasmid pCAMBIA 1304 which contains genes for hygromycin (hyg), β-gluconidase (gusA) and gfp (Fig. 2). The DHα E.coli strain ... WebSep 21, 2024 · A genetically engineered chimeric virus crTMV-CP-PLRV composed of the crucifer-infecting tobacco mosaic virus (crTMV) RNA and the potato leafroll virus (PLRV) coat protein (CP) was obtained by agroinfiltration of Nicotiana benthamiana with the binary vector pCambia-crTMV-CPPLRV. The significant levels of the chimeric virus enabled …

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WebSep 8, 2014 · In this study, a novel binary T-vector was constructed by integrating two AhdI restriction sites into the backbone vector pCAMBIA 1300. The T-vector also contains a … WebJun 1, 2024 · The new plant RNAi binary vector, named pCAMBIA2301-GW-RNAi, has two inverted repeated Gateway cassettes driven by the cauliflower mosaic virus 35S (CaMV …

WebBinary vector pCAMBIA-1300 NCBI BLAST name: other sequences Rank: species Genetic code: Translation table 1 (Standard) Plastid genetic code: Translation table 11 (Bacterial, … WebJun 9, 2024 · In the first step, an EcoRI/KpnI fragment of ~ 2.4 kb was excised from the plasmid pKSMYMIV-A and cloned into the EcoRI/KpnI sites of binary vector pCAMBIA-1300 (Cambia) to produce the recombinant plasmid pCAM-MYMIV-AM.

WebBinary vector pCAMBIA 1301 showing hptII gene near left border and GUS gene near right border of T-DNA from publication: An Efficient Protocol for the Agrobacterium-mediated Genetic Transformation ... WebpCAMBIA1300 Agrobacterium binary vector for plant transformation, with hygromycin- and kanamycin-resistance genes. Sequence Author: Cambia Open in SnapGene Try …

WebThe pBIN19 vector was developed in the 1980s and is one of the first and most widely used binary vectors. The pGreen vector, which was developed in 2000, is a newer version of …

WebNov 15, 2024 · The pCambia-1300 binary vector was used as a backbone. A pCambia–BSCTV vector was used as template to amplify pV86 or pC86 trans-activated promoters using primers pV86-GUS-R1-F/R or pC86 … neighbor\u0027s light too brightWebNov 9, 2015 · For genetic complementation, the full-length genomic DNA of the VPT1 fragment was amplified from wild type genomic DNA and cloned into the binary vector pCAMBIA-1300. To generate the VPT1VPT1 coding sequence without a stop codon, and GFP (35S:VPT1-GFP) were cloned into the pCAMBIA-1300. The tonoplast marker, … it is towards the endWebThe pCambia1301 vector offers: High copy number in E.coli for high DNA yields. pVS1 replicon for high stability in Agrobacterium. Small size. Restriction sites designed for … neighbor\u0027s meats llchttp://www.biovector.net/product/1319.html it is to you byron cage lyricsWebpCAMBIA2301 Agrobacterium binary vector for plant transformation, with kanamycin-resistance and GUS genes. Sequence Author: Cambia Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7k Plasmids: Plant Vectors More Plasmid Sets Home Plasmids Plant VectorspCAMBIA2301 Show … it is to you i give the glory chords neighbor\u0027s kitchen \u0026 yardWebAug 14, 2015 · Grains of an insect resistant transgenic Bt rice (Oryza sativa L. Japonica cv Ariete), transformed by Agrobacterium tumefaciens with the binary vector pCAMBIA 1300 containing the synthetic Cry1B gene from Bacillus thuringiensis, and its corresponding wild-type (WT) were used in this study . The CRM of the GM maize MON863 9.85 % (ERM … it is totally okay